WebFeb 1, 2013 · SCHEMA was used to design 10-block contiguous and noncontiguous recombination libraries of the three parent ChRs that minimize the libraryaverage disruption of the ChR structure (10,23, 24). Both ... X-ray diffraction data sets for the native and mercury-derivatized protein crystals were collected on beamline X06SA at SLS and beamline BL32XU at SPring-8, using a 1-μm-wide, 15-μm-high microbeam38. Data were indexed and scaled with the programs XDS39 and SCALA40, or with DENZO and SCALEPACK … See more Chimaeras between ChR1 and ChR2 from C. reinhardtii and other algal species were subcloned into the pCGFP-EU vector21 for expression in HEK293 cells. The tobacco etch virus (TEV) protease cleavage site, the … See more C1C2 was mixed with monoolein (Sigma) in a 2:3 protein to lipid ratio (w/w). Aliquots (100 nl) of the protein-LCP mixture were spotted on a 96-well … See more The cells were transfected with 0.5 μg wild-type C1C2 or C1C2 mutants, using Fugene 6, for 30 h. The cells were then washed with PBS and fixed with 4% paraformaldehyde in PBS for 20 min at room temperature … See more HEK293 cells were cultured on poly-lysine-coated, glass-bottom culture dishes (Matsunami), and were transfected with 0.5 μg of a plasmid construct containing the GFP-tagged C1C2 or the GFP-tagged C1C2 mutants. At … See more
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WebFeb 18, 2024 · Interleukin-12 (IL-12) and IL-23 are heterodimeric cytokines that are produced by antigen-presenting cells to regulate the activation and differentiation of lymphocytes, and they share IL-12Rβ1 as a receptor signaling subunit. We present a crystal structure of the quaternary IL-23 (IL-23p19/p40)/IL- … WebFeb 17, 2024 · Here, we present the 2.0 Å resolution cryo-EM structure of ChRmine, revealing architectural features atypical for channelrhodopsins: trimeric assembly, a short … WebHTML stands for Hyper Text Markup Language. HTML is the standard markup language for creating Web pages. HTML describes the structure of a Web page. HTML consists of a series of elements. HTML elements tell the browser how to display the content. HTML elements label pieces of content such as "this is a heading", "this is a paragraph", "this is ... diabetes supply storage