WebThe DNA sequence of this gene was modified by optimizing the codon usage and deleting 50 CpG motifs without changing the amino acid sequence of the wild type protein. The CpG-free Sh ble gene is provided in the bacterial plasmid pMOD2 which is selectable with Ampicillin. To facilitate the excision and subcloning of the CpG-free Sh ble gene into ...
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Limited expression of non-integrating CpG-free plasmid is ... - PLOS
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