Hieff clone plus multi one step cloning kit
Web13 de abr. de 2024 · 高保真聚合酶（Hieff Canace Plus High-Fidelity DNA Polymerase，货号 10153ES60） 一步法克隆试剂盒（Hieff Clone ® Plus One Step Cloning Kit 一步 … Webusing Hieff Clone Plus One Step Cloning Kit (Yeasen Biotech Co. Ltd.) and the vectors were transformed into EscherichiacoliDH5 cells.Sequenceswereconfirmedby DNA sequencing. The plasmids were extracted by Plasmid Maxi Preparation Kit or Plasmid Maxiprep Kit (Bey-otime, Shanghai, China), and subsequently transfected into
Hieff clone plus multi one step cloning kit
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WebGostaríamos de lhe mostrar uma descrição aqui, mas o site que está a visitar não nos permite. WebHieff Clone ® Universal One Step Cloning Kit 是新一代的同源重组克隆试剂盒，精心优化的 2×Hieff Clone ® Universal Enzyme Premix 预混了重组酶和重组反应所需缓冲液，并添加了独特的重组增强因子，可显著提高重 …
http://yeasen.com/assets/upload/file/20240907084815442.pdf WebYour search returned 43 Assay Kit across 1 supplier. Showing 30 of 43 products. 1; 2 > >> Sort By. Select All. Select up to 5 products from below to compare or request more information. Request Info for all products Compare. Viral RNA Isolation Kit Yeasen Biotechnology Co ...
WebClonExpress II One Step Cloning Kit . 非连接酶依赖型单片段快速克隆试剂盒. 快速克隆；高通量克隆；无缝克隆；DNA定点突变；双链DNA与载体组装. · 简单、快速、高效， … WebBest practices. With focuses on the research and development and manufacturing of tool enzyme raw materials, antigen and antibodies, Yeasen supplies 40+ types of enzymes, …
WebDescription. This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 ligase, …
WebDescription. The GenBuilder Plus Cloning Kit enables seamless and one-step cloning of multiple DNA fragments into a linearized vector. The proprietary enzyme mix fuses together DNA fragments efficiently and precisely by recognizing 15-40 bp overlaps at the end of each fragment. The system allows cloning of DNA fragments into any linearized ... chinese passport photo sizehttp://www.bioon.com.cn/news/showarticle.asp?newsid=68004 grand river chapter of credit unionsWeb20 de mai. de 2024 · The purified PCR product was then cloned into the pCAMBIA1300-UBI-RBCS vector using the Hieff Clone® Plus One Step Cloning Kit. The D3 knockout vector was constructed using the CRISPR/Cas9 method. Based on the genomic sequence of D3 gene, two specific CRISPR target sites were designed in the protein-encodable … chinese passport photo freeWebVazyme ClonExpress II One Step Cloning is a simple, fast, and high efficient cloning technology and enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert ... chinese passport renewal houstonWebClone High: Created by Bill Lawrence, Phil Lord, Christopher Miller. With Will Forte, Phil Lord, Michael McDonald, Christa Miller. The greatest minds of the world have been … chinese party games for kidsWeb27 de abr. de 2024 · PCR of the coding sequence of Met or Gce from the pClone007-Met or pClone007-Gce vector was performed, and then we used a Hieff Clone Plus Multi One Step Cloning Kit (10912ES10, Yeasen) for assembly. Finally, we obtained the pUAST-Met-4 × FLAG-Avi-P2A-BirA ( UAS-Met-BirA ) or pUAST-Gce-3 × FLAG-Avi-P2A-BirA ( UAS … chinese passport renewal form downloadWeb1 de jan. de 2024 · Gibson assembly was performed with Hieff Clone TM Plus Multi One Step Cloning Kit (Yeasen, Shanghai, China). The substrate ferulic acid was dissolved in H 2 O as stocking solution (800 mM) and the pH was adjusted to around 7.0 by NaOH. ... which was assembled with Gibson assembly kit, resulting in plasmid pLux-RFP. 2.2.6. grand river edges trail