Opti mem thermo
WebThermo Fisher opti mem serum free media Opti Mem Serum Free Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. WebProduct name Opti-MEM® I Reduced-Serum Medium (1X), liquid Page 4 / 8 None None Chemical Name Korea OEL (CEILING) 18-Aug-2015 Korea OEL (TWA) Korea OEL (STEL) www.thermofisher.com None Always wear recommended Personal Protective Equipment. Wear personal protective equipment. Product code Ammonium metavanadate 7803-55-6 …
Opti mem thermo
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WebOpti-MEM™ I Medium (Tube 2) – Mix well Opti-MEM™ I Medium 5 μL 25 μL 125 μL Lipofectamine ™ CRISPRMAX Reagent 0.3 μL 1.5 μL 7.5 μL 4 5 Incubate Incubate Cas9 nuclease/gRNA/Cas9 Plus™ solution (Tube 1) and diluted CRISPRMAX™ Reagent in Opti-MEM™ Medium (Tube 2) for 5 minutes at room temperature. 5 Tube 1T ube 2 Prepare … WebOpti-MEM I medium 25 µL Lipofectamine 3000 reagent 2 µL 2 Tube 2 Opti-MEM I medium 25 µL DNA amount (DNA concentration should be 0.5–5 µg/µL) 250 ng P3000™ reagent 0.5 µL 3 Add tube 2 solution to tube 1 and mix well 4 Incubate mixture from step 3 at room temperature for 10–15 min 5 Add 50 µL of complex from step 4 to cells;
Webnepa21 高效基因转染系统(日本nepa gene)；olympus ix51倒置荧光显微镜(奥林巴斯)； nanodrop 2000超微量分光光度计(gene company limited)；galliostm流式细胞分析仪(beckman coulter)；varioskan flash全波长光谱扫描仪(thermo scientific)；js-680d全自动数码凝胶成像分析系统(上海培清科技有限 ... WebFeb 12, 2024 · Opti-MEM (Thermo Fisher Scientific, Waltham, MA, USA). 8. Stereomicroscope. 9. Glass capillary pipettes. 10. Fluorescent inverted microscope. 11. ... in Opti-MEM medium. 3. Add 100 μg/mL of ssODN in the nuclease solution when produce knock-in strain. 4. Introduce 10 μL of nuclease solution into the electrode (Fig. 1b). 5.
WebMar 10, 2024 · BY-2-xGxFL cells were collected in a tube and washed once with Opti-MEM I (Thermo Fisher Scientific, Waltham, MA, USA). Cells with 20 µL of PCV were suspended in … WebApr 12, 2024 · For transfection, HEK293 cells were washed with Opti-MEM Reduced Serum Media (Gibco) and incubated with transfection mix containing 1 µg total of the indicated plasmid DNA and 3 µl Lipofectamine ...
WebItem Opti-MEM™ I Reduced Serum Medium Company Thermo Fisher Scientific Price Pricing Info Get Quote Get Quote for this Product Catalog Number 31985062 Quantity 100 mL Applications CELL CULTURE Regulatory Status For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals Type Medium
WebOpti-MEM™ I Reduced Serum Medium (Cat. no. 31985) ∤ Eppendorf tubes Timing ∤ Preparation: 10 minutes ∤ Incubation: 5 + 10 minutes ∤ Final Incubation: 2–3 days … how to screen record on a computer dellWebBe the first to write a review! Opti-MEM Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine ... how to screen record on acer computerWebTransfection reagents were diluted by opti-MEM (Thermo Fisher Scientific) for 5 min at room temperature (RT). Plasmids were also diluted by opti-MEM. Then the diluted … north plains gunworksWebMar 31, 2024 · CSII-EF-IN was converted to a Gateway destination vector by the insertion of the RfA cassette (11828029, Thermo Fisher Scientific). MYADM cDNAs were transferred from pENTR to CSII-EF-IN using LR Clonase (11791020, Thermo Fisher Scientific). pBluescript II SK (+) was used as the cloning vector (212205, Agilent). how to screen record on a computer with soundWebSep 30, 2024 · All siRNAs used in this study were obtained from RIBO Biotechnology (China) and transfected into CCA cells using Lipotransfectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA) according to the instructions. Briefly, CCA cells were seeded in a six-well plate until the cell density reached approximately 60%. 200 μL serum-free OPTI-MEM ... how to screen record on a computerWebOpti-MEM ™ I Reduced Serum Medium. Opti-MEM ™ I Reduced Serum Medium is a serum-free medium used to complex plasmid DNA with ExpiFectamine ™ 293 Reagent, providing … north plains loyal ridersWebOpti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO 2 environment to maintain physiological pH. cGMP manufacturing … Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes north plainfield howard johnson